Detection of Flavonoid Compounds of Daruju Root Extract (Acanthus ilicifolius Linn) using Thin Layer Chromatography and UV-Vis Spectrophotometry

Aden Dhana Rizkita (Sekolah Tinggi Ilmu Kesehatan Bogor Husada)
Ilham Maulana (Program Studi S-1 Farmasi, Sekolah Tinggi Ilmu Kesehatan Bogor Husada, Bogor, Indonesia)
Sintia Ayu Dewi (Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang, Semarang, Indonesia)

Jurnal Sains dan Kesehatan
Vol. 5 No. 1 (2023): J. Sains Kes.

The isolation and detection of flavonoids in the extract of Daruju root has been carried out. extract obtained from maceration modification using 96% ethanol, ethanol extract The extraction result is concentrated with a rotary evaporator and then evaporated above water bath until a thick extract is obtained. The resulting extract was further separated using a separating funnel and diethyl ether and n-butanol as solvents. Then the thick extract was tested for plavonoid preliminaries by reacting the thick extract with H2SO4 and Mg the results obtained were a change in color to yellow, it proved that the extract was positive for plavonoid. After that, TLC was carried out to purify the extract which would later detect plavonoid using a UV-Vis spectrophotometer. TLC results show an Rf value of 0.6-0.8. UV-Vis spectrophotometer results at a wavelength of 200-800 nm give results in fraction A with a maximum wavelength at 291 nm which is suspected to have detected flavonoids.