Jurnal Perikanan dan Kelautan
Vol. 28 No. 1 (2023): February

Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter)

Bintang, M. Irwan Sapta Purna (Unknown)
Sukmiwati, Mery (Unknown)
Karnila, Rahman (Unknown)

Article Info

Publish Date
24 Jan 2023

Abstract

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin.

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Journal Info
Jurnal Perikanan dan Kelautan
Website

Abbrev

jpk

Publisher

Universitas Riau

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy Materials Science & Nanotechnology Physics

Description

Jurnal Perikanan dan Kelautan (JPK) aims to promote interdisciplinary research and to foster collaboration among researchers from diverse backgrounds. Jurnal Perikanan dan Kelautan (JPK) is committed to publishing high-quality, original research that makes a significant contribution to fields of ...