Experimental comparative study and fracture resistance simulation with irrigation solution of 0.2% chitosan, 2.5% NaOCl and 17% EDTA

Ernani Ernani (Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara)
Tri Murni Abidin (Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara)
Indra Indra (Department of Mechanical Engineering, Faculty of Engineering, Universitas Sumatera Utara)

Dental Journal (Majalah Kedokteran Gigi)
Vol. 48 No. 3 (2015): September 2015

Background: Preparation in endodontic need irrigation materials as root canal debridement and disinfectant. However, irrigation materials is one of the factors that influence the tendency of fracture. Purpose: This study was aimed to see the resistance and fracture distribution if teeth irrigated with high molecular horseshoe crab chitosan at 0.2% concentration, 2.5% NaOCl solution and 17% EDTA solution in endodontic treatment with finite element method (FEM) simulation study and experimental studies. Method: Endodontic treatment performed on 28 maxillary premolars with group A: irrigation solution of 17% EDTA and 2.5% NaOCl solution; group B: 2.5% NaOCl irrigation solution; group C: 2.5% NaOCl irrigation solution and 0.2% chitosan solution; group D: 0.2% chitosan solution irrigation. Final restoration was done using prefabricated glass fiber post. Cementation of post using resin cement then restored with direct composite resin restorations. Pressure test was performed with a Universal Testing Machine with a speed of 0.5 mm/min until fracture occurred. A three dimensional finite element analysis was performed for total deformation, equivalent (von-mises) stress, and equivalent elastic strains. Result: Anova test showed significant differences in fracture resistance (p<0.05) in stroke difference between four groups. Based on the results of the analysis of post-hoc Bonferroni test and LSD, fracture resistance was significantly different between group A (1038.4±201.6) with group C (1515.6±243.3). Kruskal-Wallis test showed no significant difference in the distribution of fractures among the four groups (p>0.05). Statistical analysis showed no significant difference (p>0.05) between the results of experiment and FEM analysis results using the t-test. Conclusion: The results of this study demonstrated that there was effect of the use of high molecular 0.2% chitosan as a combined irrigation with NaOCl, but did not affect the fracture pattern distribution of endodontically treated teeth both experimentally and FEM analysis test.