<![CDATA[Pangasianodon hypopthalmus can be reared under biofloc system and short photoperiod. To understand the effect of biofloc system and photoperiod manipulation to wards blood structure of P. hypopthalmus a study was conducted on September to December 2020. This experiment using a factorial CRD design with 2 level for B factor (biofloc and no biofloc), 3 level for P factor (Natural, 18 hours dark, 24 hours dark), 3 repetition. The fish was reared in 110L tank, 30 fishes/tank, fed with fish pellet (FF-999, Provit 781-1 and Provit 781-2). Parameters measured were the number of erythrocytes, leukocytes, the level of hematocrit and leukocrite and leukocyte types. The blood was collected using syringe wetted with EDTA 10%. The number of blood cells was calculated using haemocytometer, while the white blood cells were stained using Giemsa solution and checked under a binocular microscope. Results shown that there is no different blood structure in fish of all treatments. Total erythrocytes are 1,681,111-2,647,777 cell/mm3, total leukocytes are 55.031-55.655 cell/mm3, hematocrit 20.33-38.11% and leukocrite 20.33-38.11%. White corpuscle are basophil 00-3.89%, eosinophils 0-3.67%, lymphocytes 26.67-73.89%, trombosit 1-48.89%, monocytes 13.33-30.22% and neutrophill 0.00-9.22%. Based on data obtained, it can be concluded that the biofloc system and photoperiod manipulation combination do not negatively affects the fish hematology.]]>
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