Jurnal Ilmiah Sains
Volume 24 Issue 2, October 2024

Effect of Phosphate Source on Growth of Chlorella vulgaris Cultured with Indole-3-Acetic Acid-Producing Bacteria

Wijaya, Lintang Guritno Cucu (Unknown)
Prabaningtyas, Sitoresmi (Unknown)



Article Info

Publish Date
17 Oct 2024

Abstract

The need for energy sources in Indonesia continues to increase, in 2018 Indonesia is a country with energy consumption of around 114 Million Tons of Oil Equivalent (MTOE). Therefore, alternative energy from biomass such as biodiesel is needed. Chlorella vulgaris is a microbe suitable for alternative energy because it contains up to 80% lipids. Chlorella vulgaris needs phosphorus for cell growth from fertilizer for the production of Adenosine Triphosphate (ATP). In addition to phosphorus, co-cultures with IAA-producing bacteria can promote the growth of C. vulgaris.  This study was conducted to evaluate the influence of phosphate sources on the growth  of C. vulgaris cultured with IAA-producing bacteria. This study was carried out by co-culture C. vulgaris and IAA-producing bacteria with different phosphate sources on Gusrina medium for 27 days. The treatment consisted of treatment A (control), B Triple Super Phosphate (TSP), and C Super Phosphate-36 (SP-36). Cell growth was calculated using  the Petroff-Hausser counting chamber method  followed by the ANOVA test. The highest cell growth was obtained in treatment B (TSP), followed by treatment C (SP-36), and finally treatment A (control). The results showed that the addition of phosphate sources had an effect on the growth  of C. vulgaris with the highest number of cells found in treatment B (TSP) of 2,66 × 106 cells/mL. Keywords: Chlorella vulgaris; co-culture; phosphorus

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Journal Info

Abbrev

JIS

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics

Description

Jurnal Ilmiah Sains (Journal of Scientific Sciences) is the Journals Published by Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. Jurnal Ilmiah Sains Published Twice a Year, i.e April and October. Jurnal Ilmiah Sains  welcomes full research articles in the area of ...