Journal of Multidisciplinary Applied Natural Science
Vol. 4 No. 1 (2024): Journal of Multidisciplinary Applied Natural Science

Antioxidant and Antibacterial Activities of Curd and Whey Kefir Produced from Etawa Goat Milk

Siwi Meutia Sadewi (Magister of Chemistry Program, Lampung University, Bandar Lampung-35141 (Indonesia))
Nurhasanah Nurhasanah (Department Chemistry, Lampung University, Bandar Lampung-35141 (Indonesia))
Sudibyo Sudibyo (Research Center for Mining Technology, National Research and Innovation Agency (BRIN), South Lampung-35352 (Indonesia))
Neneng Windayani (Department of Chemistry Education, UIN Sunan Gunung Djati, Bandung-40614 (Indonesia))
Agung Abadi Kiswandono (Department Chemistry, Lampung University, Bandar Lampung-35141 (Indonesia))
Heri Satria (Department Chemistry, Lampung University, Bandar Lampung-35141 (Indonesia))



Article Info

Publish Date
11 Dec 2023

Abstract

Kefir is a fermented drink with many benefits. Kefir contains antioxidant compounds that can inhibit free radical activity.In addition, kefir contains lactic acid bacteria, which provide good antibacterial activity and improve the health of the digestive tract. This research aimed to determine the antioxidant and antibacterial activities of kefir made from Etawa goat milk. The methods used in this research included fermentation of Etawa goat milk with 10 % kefir seeds at 37 °C and a fermentation time of 2 days. Antioxidant activity was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and antibacterial activity was tested using agar diffusion. The peptide molecular weights in kefir were analyzed using SDS-PAGE. The results showed that the antibacterial and antioxidant activities in Etawa goat's milk curd kefir has a higher activity than whey kefir. The antioxidant activity of curd has an IC50 of 43.99 ppm, which is a powerful antioxidant. Curd's antibacterial activity forms a clear zone of 7 mm against Bacillus cereus and is included in the solid antibacterial category. In Escherichia coli, a clear zone of 6 mm was formed and included in the moderate antibacterial category. The results of SDS-PAGE gel visualization found three prominent bands measuring 10, 15, and 33 kDa, respectively.

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Journal Info

Abbrev

jmans

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Description

Journal of Multidisciplinary Applied Natural Science (abbreviated as J. Multidiscip. Appl. Nat. Sci.) is a double-blind peer-reviewed journal for multidisciplinary research activity on natural sciences and their application on daily life. This journal aims to make significant contributions to ...