This study assessed mothers' knowledge about immunization and the factors influencing it through a descriptive study conducted from April to November 2020 on 160 randomly selected women in Diwaniyah. A questionnaire was used to collect demographic, social, and economic data, as well as awareness of vaccine-preventable diseases and vaccination records from 10 primary healthcare centers. Data were analyzed using SPSS 23 with descriptive and inferential statistical methods. Results showed that 52.5% of mothers had moderate knowledge about mandatory vaccinations, while a quarter had low knowledge, indicating a significant lack of awareness. Factors such as age, education level, and marital status greatly influenced their knowledge, with younger and less-educated mothers showing lower awareness. The study recommends awareness campaigns, distribution of informative materials, and intensified vaccination efforts for healthcare workers to address dropouts. Highlights: Assess mothers' knowledge of immunization and influencing factors. Descriptive study, 160 participants, questionnaire, SPSS analysis. Moderate knowledge, influenced by age and education; recommend awareness campaigns. Keywords: Mothers' Knowledge, Immunization of One-Year-Old
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