Dinamika Pertanian
Vol. 40 No. 2 (2024): Jurnal Dinamika Pertanian Edisi Agustus 2024

ANALISIS KEANEKARAGAMAN GENETIK TIGA POPULASI MATOA (Pometia pinnata Forst & Forst) BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA)

Dwi Wulan (Unknown)
Dewi Indriyani Roslim (Unknown)
Adiwirman (Unknown)



Article Info

Publish Date
06 Feb 2025

Abstract

Matoa is a plant belonging to the Sapindaceae family, commonly found in tropical regions such as Indonesia. It produces fruit that is renowned for its unique flavor, which is a delightful blend reminiscent of longan, rambutan, and durian, giving it significant economic value. The skin of the matoa fruit comes in various colors, including yellow, green, red, and purple. This study aims to evaluate the genetic diversity among three matoa populations using RAPD (Random Amplified Polymorphic DNA) markers. The populations analyzed include three from Kab. Kampar and two from Pekanbaru City, utilizing four RAPD primers in the process. The research involved multiple steps: collecting leaf samples, isolating total DNA, conducting total DNA electrophoresis, amplifying DNA with RAPD markers, electrophoresing the PCR products, scoring the RAPD bands, and analyzing the data. The findings revealed that the four RAPD primers generated a total of 65 DNA bands, with sizes ranging from 250 bp to 3100 bp. The genetic diversity values among the matoa genotypes varied between 8.748% and 16.186%. Clustering analysis based on the four RAPD primers indicated that the tested matoa samples were not distinctly grouped according to the color of their fruit peels or their geographical locations.

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Journal Info

Abbrev

dinamikapertanian

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Immunology & microbiology

Description

Dinamika Pertanian is a scientific journal published by the Faculty of Agriculture Islam University of Riau Pekanbaru three times a year (April August and December). Journal publishes new articles and original research on topics related to agriculture broadly. This journal accommodates articles ...