Jurnal Ilmiah Kedokteran Wijaya Kusuma
2009: edisi khusus Desember 2009

ISOLASI DAN KARAKTERISASI ENZIM SELULASE

masfufatun, masfufatun (Unknown)



Article Info

Publish Date
30 Sep 2017

Abstract

Carboxy Methyl Cellulose (CMC) is a derivative of cellulose soluble in water. Therefore, CMC easily hydrolyzed into simple sugars by the enzyme cellulase. Snails are animals soft, easy to breed and utilize cellulose as a source of energy and protein content is high enough. Therefore, snails can be used as a source of cellulase enzyme to hydrolyze carboxy Methyl Cellulose (CMC) This research aims to isolate cellulase enzyme from the snail, Achatina fulica and determine itscharacterization. Glucose levels produced from cellulose enzyme activity were analyzed by using the method Semogy-Nelson. From this research it turns out cellulase enzyme isolated from hepatopankreas snail, Achatina fulica has a specific activity of 2.85 U / mg protein and activity 50oCdan temperature optimum at pH 5.16 and have had kinetic parameters Vm price of 0.23 mg / mLper minute and Km is 0.53 mg / mL. Part enzyme cellulose getting fed at a concentration of 4%.

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Journal Info

Abbrev

JIKW

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

Description

Jurnal Ilmiah Kedokteran Wijaya Kusuma (JIKW) is a periodically scientific publication that contains articles written in Indonesia or english. JIKW receive articles in the scope of Biomedical Sciences, degenerative diseases, infections, congenital abnormalities and public health. JIKW published ...