Cyclic Voltammetry Study of the Influence of Concentration of K3[Fe(CN)6] and Glucose on Glassy Carbon Electrode

Adella Fajaretta Gunawan (Jenderal Achmad Yani University Jalan Terusan Jenderal Sudirman, Cimahi Selatan, Cimahi, Jawa Barat 40513, Indonesia)
Arie Hardian (Jenderal Achmad Yani University Jalan Terusan Jenderal Sudirman, Cimahi Selatan, Cimahi, Jawa Barat 40513, Indonesia)
Anceu Murniati (Jenderal Achmad Yani University Jalan Terusan Jenderal Sudirman, Cimahi Selatan, Cimahi, Jawa Barat 40513, Indonesia)

ALCHEMY Jurnal Penelitian Kimia
Vol 21, No 1 (2025): March

Biosensors are cost-effective, user-friendly devices with rapid response, sensitivity, selectivity, and stability. This study investigated the impact of K3[Fe(CN)6] concentration and glucose on glassy carbon electrode (GCE) performance. The cyclic voltammetry method determined the effects of varying K3[Fe(CN)6] and glucose concentrations on GCE. GCE characterization utilized K3[Fe(CN)6] solutions (1 – 5 mM) in 0.2 M KCl and achieved an optimum scan rate of 75 mV/s within -1.5 to 1 V vs. Ag/AgCl. Reversible Fe2+/Fe3+ redox occurred at 1mM (ipa/ipc= 0.99), with the highest reduction peak at 5mM. Glucose tests (1mM to 5mM) in 0.1 M NaOH exhibited an optimal scan rate of 200 mV/s within a potential range of -10 – 10 V at 1 mM (ipa/ipc= 1.01), with the highest oxidation peak at 5 mM. GCE effectively measured concentration effects, with ipa/ipc values close to 1, indicating reversible redox processes at low concentrations. Higher concentrations yielded stronger peak currents due to increased electroactive species.