<![CDATA[Pacar air flowers (Impatiens balsamina L.) hold potential as natural antibacterial agents, rich in compounds like flavonoids, alkaloids, steroidal saponins, tannins, quinones, and phenols. Pacar air flowers are utilized in traditional medicine for various medical conditions, including antibacterial properties. The purpose of this study is to determine the effect of using balsam flower extract (Impatiens balsamina L.) as a natural antiseptic in reducing total plate count on the hands of Poltekkes Denpasar students. Sterile hand swab samples were collected using a Pretest-Posttest Control Group Design to assess differences before and after the application of various concentrations. Statistical analysis was performed using One-way ANOVA. The study revealed differences in total plate count before and after the application of pacar air flower extract (Impatiens balsamina L.) on the hands of Poltekkes Denpasar students. The mean total plate count before treatment was 29,98 CFU/cm2, while after treatment, it was 4,70 CFU/cm2. Total plate count significantly decreased (p0.05) after applying the pacar air extract compared to before (Impatiens balsamina L.). There was a significant reduction in total plate count with the application of extract concentrations of 5%, 10%, and 15%]]>
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