The purpose of this study was to evaluate the microorganisms found on used toothbrushes. In this study 25toothbrushes were investigated, 10 toothbrushes were used less than 3 months, 10 toothbrushes have been used for3-6 months, and 5 toothbrushes have been used for more than 6 months. Some gram-positive coccus, such asStaphylococcus, Streptococcus and Diplococcus. The results showed there were microbial harbored of usedtoothbrushes. Toothbrushes that have been used for 6 months were harbored more microorganisms than thetoothbrushes that have been used less than 3 months and 3-6 months. It can be concluded that the longer using timeof toothbrush the more increase of microorganism amount found on toothbrush.
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