Biogenesis: Jurnal Ilmiah Biologi
Vol 12 No 2 (2024)

Staphylococcus aureus contamination in traditional Balinese Indonesian stem food: Tum bungkil, Tum pusuh and Tum biu batu

Kusumaningsih, Purwaningtyas (Unknown)
Puspaningrum, Dylla Hanggaeni Dyah (Unknown)
Dachi, Milafebriani (Unknown)
Mboru, Adriana Dehi (Unknown)



Article Info

Publish Date
02 Aug 2025

Abstract

Tum is a traditional Balinese Indonesian dish made from various parts of the stone banana tree, including the stone banana root bulb (bungkil), stone banana flower (pusuh), and stone banana fruit (biu batu). However, when tum is made by hand without gloves, there is a risk of contamination with Staphylococcus aureus. Although the steaming process may reduce some bacteria, contamination can still occur. S. aureus can survive in ready-to-eat foods prepared under poor hygiene conditions. The toxins produced by S. aureus can lead to skin infections, respiratory problems, and sepsis. This study aims to investigate the presence of S. aureus in processed tum. Purposive sampling was conducted in two districts that sell tum bungkil, pusuh, and biu batu. Six samples were tested using mannitol salt agar (MSA) which is specific media from a dilution level of 10-3. Identification is conducted based on morphology, gram staining and total plate count (TPC) results. The results indicated that the highest level of contamination was found in sample 3 (S3) were 1.7x102CFU/g, which contained tum pusuh. This study implies that it is essential to improve hygiene practices during the preparation of tum pusuh to minimize the risk of contamination.

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Journal Info

Abbrev

biogenesis

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic ...