Journal of Global Pharma Technology
Volume 16 Issue 04 (2024) April 2024

BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF OXACEPROL IN HUMAN PLASMA BY USING UV-VISIBLE SPECTROPHOTOMETER

Sri, K. Bhavya (Unknown)
Banu, Shaheen (Unknown)
Sumakanth, Mogili (Unknown)



Article Info

Publish Date
01 Apr 2024

Abstract

Osteoarthritis is commonly treated with oxaceprol. An easy, quick, accurate, and exact technique for estimating oxaceprol is required since 1 billion people worldwide suffer from rheumatoid arthritis each year. Utilizing a UV-visible spectrophotometer, procedures are developed and then validated. Only a few techniques for estimating oxaceprol have been described, but after reviewing the literature, we developed a method with a wide linearity range and low DL and QL values. Methanol: ACN was used as the solvent in the development of this procedure. The chosen wavelength for examination in this approach was discovered to be 215nm. To do the spectrum study, a double beam spectrophotometer with UV and visible light was employed.In the UV spectrophotometer, the absorbance of several series of dilutions was measured at a wavelength of 215 nm. Oxaceprol concentration was plotted on x-axis & absorbance was taken on y-axis to plot Calibration curve. All the validation parameters were validated as per ICHQ2 (R1) guidelines. Keywords: Osteoarthritis, UV-visible spectrophotometer, linearity, calibration curve, methanol.

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Journal Info

Abbrev

jgpt

Publisher

Subject

Medicine & Pharmacology

Description

ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and ...