Biotransformasi 2E-6E-Farnesol oleh Jamur Endofit Botryosphaeria sp. CA2C-3 yang Diisolasi dari Temu Hitam (Curcuma aeruginosa ROXB.)

Agusta, Andria

JURNAL BIOLOGI INDONESIA
Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA

The objective of study was to investigate the microbial transformation reaction of 2E-6E-farnesol by the endophyticfungi isolated from temu hitam (Curcuma aeruginosa ROXB. The transformation was carried out in PDB with (2E-6E-farnesol), incubated at room temperature (25-32o C) under shaking condition at 120 rpm for two days produceda major biotransformed product. Structure elucidation based on 1D- and 13C-NMR analysis showed that the biotransformedproduct was 10,11-dihydroxi-2E-6E-farnesol. It It was verified that biotransformation reaction of 2E-6E-farnesol into 10,11-dihydroxi-2E-6E-farnesol through an intermediate 10,11-epoxi-2E-6E-farnesol.Keywords: Temu hitam; Curcuma aeruginosa; endophytic fungi; biotransformation; 2E,6E-farnesol; 10,11-dihydroxi-2E,6E-farnesol; 10,11-epoxi-2E-6E-farnesol.