Jurnal Ilmiah Sains
Volume 25 Issue 1, April 2025

Flavonoid Levels in Beluntas Leaves (Pluchea indica (L.) Less) in Lamongan Regency Using a Spectrophotometer UV-Vis

Faizatul Fitria (Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Indonesia)
Farida Noor Arifah (Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Indonesia)
Prima Agusti Lukis (Faculty of Dentistry, Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Indonesia)
Sofira Dwi Meylinda Sari (Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Indonesia)



Article Info

Publish Date
30 Apr 2025

Abstract

The beluntas plant (Pluchea indica (L.) Less) is one of the Indonesian medicinal plants that is rich in secondary metabolite compounds, one of which is flavonoids. The level of flavonoid compounds in beluntas leaves (Pluchea indica (L.) Less) is quite large, around 2.79%. Flavonoids in beluntas leaves provide the greatest biological activity at 63.1%, and can be used for health. The purpose of this study is to determine the total flavonoid levels of beluntas leaf extract from Lamongan regency using Spektrophotometer UV-Vis with quersetin as standart. The extraction method used is maceration with 70% ethanol solvent and the ratio of simplicia and solvent is (1:5). The presence of flavonoid compounds was determined by qualitative tests using concentrated HCl reagents and Mg powder. Total flavonoid levels were analyzed using a UV-Vis spectrophotometer with a quercetin standard measured at a wavelength of 408 nm for 2 minutes. Positive results are shown from qualitative tests by a change in orange color. The beluntas leaf extract obtained was 56.024 grams with a yield of 14.006%. The total flavonoid content from measurements using a UV-Vis spectrophotometer was 6.522%. Keywords: Beluntas leaves; flavonoids; maceration; secondary metabolites; Spectrophotometer UV-Vis

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Journal Info

Abbrev

JIS

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics

Description

Jurnal Ilmiah Sains (Journal of Scientific Sciences) is the Journals Published by Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. Jurnal Ilmiah Sains Published Twice a Year, i.e April and October. Jurnal Ilmiah Sains  welcomes full research articles in the area of ...