Jurnal Bios Logos
Vol. 15 No. 3 (2025): JURNAL BIOS LOGOS

Development of Commercial Sterility Detection Method Based on Chromogenic Media Agar in UHT Coconut Cream Liquid Products

Ester Ruth Youla Tangkau (Department of Quality, PT Sasa Inti, South Minahasa)
Chindy Achika Rori (Department of Quality, PT Sasa Inti, South Minahasa)
Yoel William Prasetyo Kamto (Department of Quality, PT Sasa Inti, South Minahasa)
Brayen Mangangantung (Department of Quality, PT Sasa Inti, South Minahasa)
Agustina Monalisa Tangapo (Department of Biology, FMIPA, Universitas Sam Ratulangi)



Article Info

Publish Date
23 Nov 2025

Abstract

Commercial sterility analysis is a crucial step in quality control of Ultra High Temperature (UHT) products such as coconut cream liquid products produced by PT Sasa Inti Minahasa Selatan, to ensure the safety and stability of shelf life. Stability incubation testing takes a long time, which is 7-14 days. Development of commercial sterility methods using Chromogenic Agar selective media is needed to cut the incubation time that is too long. Agar Chromogenic Media is tested for its ability to detect common contaminants in UHT coconut cream products, in particular thermophilic and mesophilic microorganisms that cause spoilage, with a short incubation time. UHT coconut cream samples are prepared by artificial and natural inoculation. Validation was carried out by comparing the results on standard media based on sensitivity, specificity and Time-to-result (TTR) parameters. The results showed a real difference between NA/TGEA media and chromogenic media so that the reading of bacterial colonies growing on chromogenic media was clearer and easier to read due to the change in color and short incubation time where the bacteria causing decay had grown on day 5 with a reading of results that took only 24 hours. Based on the validation that has been carried out, chromogenic selective media can accelerate product release time and improve quality control efficiency in the UHT coconut cream industry.

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Journal Info

Abbrev

bioslogos

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ...