<![CDATA[Background: Burns are skin injuries caused by heat exposure that can damage the epidermis, dermis, and even the hypodermis, while also affecting connective tissue, muscle, cutaneous nerves, and blood vessels, thereby disrupting the body's homeostasis. Objective: This study aimed to determine the effect and effective concentration of ethanol extract of skipjack bone (Katsuwonus pelamis) on the epithelialization capacity of burn-induced rats. Methods: Twenty-five rats were divided into five groups: a negative control (KN, distilled water), a positive control (K+, 0.9% NaCl), and three treatment groups (K1, K2, K3) given ethanol extract of skipjack bone at concentrations of 40%, 60%, and 80%. Second-degree burns were induced using a 2 mm iron plate heated to 60°C and applied for 30 seconds on the dorsal skin of the rats. The test preparations were applied topically twice daily for 14 days; erythema score and wound morphology were recorded daily. This was a true experimental study using a pretest-posttest control group design, analyzed using one-way ANOVA and the LSD post-hoc test. Results: One-way ANOVA showed no significant difference in erythema score among the five groups on day 14 (F(4,20) = 0.529; p = 0.716), and the LSD post-hoc test found no significant difference between any pair of groups. Nevertheless, macroscopic observation showed that the 80% concentration group (K3) exhibited earlier scar tissue resolution and epithelial regeneration by day 14 than the 40% and 60% groups. Conclusion: Ethanol extract of skipjack bone showed an indicative effect on the macroscopic appearance of burn wound healing, but did not significantly alter erythema score at the sample size used in this study (n = 5 per group). The 80% concentration showed the most pronounced descriptive trend and warrants further testing with a larger sample size and histopathological examination.]]>
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