Mastitis is an inflammatory response caused by microorganisms or physical trauma that occurs in the udder. This study used an epidemiological survey approach in which the prevalence of subclinical mastitis and the factors that affect subclinical mastitis were calculated. The subclinical mastitis test uses indirect tests with California mastitis test reagents and direct tests with breed test methods. Risk factors for hygiene and bedding materials were identified via questionnaire aids. Data processing was performed via descriptive data and the chi-square test followed by relative risk correlation. The results of the present study revealed that the prevalence of subclinical mastitis in the Karanganyar milk shelter 2 cooperative of the Tani Makmur village unit, Lumajang, was 25.2% in the California mastitis test and 30.08% in the breed test. Cages made of nonrubber (p<0.05; relative risk [RR] = 7.519; 95% confidence interval (CI): 3,953–14,298]) in the breed test and (p<0.05; relative risk [RR] = 5.9; 95% confidence interval (CI): 3,019–11,559]) in the California mastitis test. Cleanliness with a gross value has (p<0.05; relative risk [RR] = 3.701; 95% confidence interval (CI): 2,360–5,803]) in the breed test and (p<0.05; relative risk [RR] = 4.01; 95% confidence interval (CI): 2,3576,788]). This is because materials that have a rough surface can increase the degree of trauma to the udder. In summary, the dirtier cage presented a higher rate of subclinical mastitis infection than did the clean cage. Dirty cages can be a place for the growth of pathogenic microorganisms that are detrimental to livestock.
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