Short communication: Disparities in early luteal phase progesterone concentrations between repeat breeder and fertile Aceh cattle

CUT NILA THASMI (Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
UMAR PUTEH HAFIZUDDIN (Syiah Kuala University)
HUSNURRIZAL HUSNURRIZAL (Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
TONGKU NIZWAN SIREGAR (Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
HERRIALFIAN HERRIALFIAN (Laboratory of Biochemistry, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
AMALIA SUTRIANA (Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
ROSMAIDAR ROSMAIDAR (Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
FARAH FARIDA HANUM (Department of Clinical Veterinary, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
GHOLIB GHOLIB (Laboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
ARMAN SAYUTI (Laboratory of Clinic, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, No. 4, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia)
YUSMADI YUSMADI (Department of Animal Science, Faculty of Agriculture, Universitas Almuslim. Jl. Almuslim Matangglumpangdua, Peusangan, Bireuen 24261, Aceh, Indonesia)
RINA AULIA BARUS (Livestock Breeding and Forage Centre of Indrapuri (BPTU-HPT). Reukih Dayah, Indrapuri, Aceh Besar 23952, Aceh, Indonesia)
MOHAMMED AHMED ELSHAREF ABDELBAGI (Faculty of Animal Production, University of Khartoum. Khartoum 11115, Sudan)

Asian Journal of Agriculture
Vol. 9 No. 2 (2025)

Abstract. Thasmi CN, Hafizuddin UP, Husnurrizal, Siregar TN, Herrialfian, Sutriana A, Rosmaidar, Hanum FF, Gholib, Sayuti A, Yusmadi, Barus RA, Abdelbagi MAE. 2025. Short communication: Disparities in early luteal phase progesterone concentrations between repeat breeder and fertile Aceh cattle. Asian J Agric 9: 854-860. Repeat breeding remains a major reproductive constraint in cattle, often associated with altered luteal function and inadequate progesterone production during early estrus cycles. Understanding hormonal differences between fertile and repeat breeder cows is essential for improving reproductive efficiency in Aceh cattle (Bos indicus). This study aimed to investigate disparities in progesterone concentrations during the early luteal phase between repeat breeder and fertile Aceh cows. Six multiparous Aceh cows, aged 3-5 years and weighing 150-250 kg, were selected as study subjects. The cows were divided into two groups: repeat breeders (n=3) and fertile cows (n=3). To ensure synchronized estrus timing, all cows received 5 ml PGF2α (Enzaprost®-T, Dinoprost 5 mg) using the by double injection method. Estrus detection was performed following PGF2α injection in the morning and afternoon. Blood samples were collected on days 3 and 6 post-estrus for progesterone analysis. Progesterone concentrations were determined measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method, and the data were analyzed using the Mann-Whitney U test. Results indicated that progesterone concentrations on days 3 and 6 were 5.68±4.98 and 6.61±3.04 ng/mL in fertile cows, whereas in repeat breeder cows, the concentrations were only 0.35±0.07 and 2.74±0.58 ng/mL, respectively. A significant difference in progesterone concentration was observed on day 3 between the two groups (p < 0.05). The conclusion indicates that repeat breeder Aceh cows exhibit lower progesterone concentrations during the early luteal phase compared to fertile cows. These findings contribute significantly to the understanding of hormonal differences between the two groups, which may serve as a foundation for developing more effective and sustainable breeding strategies.