<![CDATA[Several health conditions are thought to be associated with monosodium glutamate (MS.G). Nowadays, a lot of products depend on monosodium glutamate as a flavor enhancer and food additive. The usage of MS.G is still debatable, even though it has been considered a safe food additive. A delayed excitotoxic and tissue-damaging dietary additive, MS.G, can produce large quantities of free radicals that cause metabolic disturbance and damage several organs. The study aimed to investigate the impress of monosodium glutamate in the testis of mice and the significance of Shilajit (Shi) in improving the damage. Fifty male albino mice weighing between 18–22g were used in the study. The animals were randomly selected into five groups. GroupA received distilled water as a control, groupB give (2g/ kg) from (MS.G), and group C gives (2g/ kg) of (MS.G) and (100mg\ kg) of Shilajit (Shi), group D gives (4g/ kg) of (MS.G), and groupE gives (4g/ kg) of (MS.G) and (200mg/ kg) of Shilajit (Shi), orally for 2 weeks. Blood collected for hormones biochemical examination. The mice were sacrificed at the end of the experiment, and the testes were separated for histological study. The results showed a significant decrease in testis weight in groups B, C, and D compared to the control, while no change in group E. The results indicated, a significant decrease at the FSH and LH. While, testosterone and progesterone levels showed a decrease in groups B, C, and D, but groupE was normal. The capsule thickness showed a significantly increased in the tested groups, except group E appeared relatively normal. Numerous histological alterations in the testis were discovered by the MS.G groups, including disarray of seminiferous tubules, decreased sperms in the lumen, Hyalinization with necrosis of Leydg’s cells and destruction of intermediate connective tissue cells, increased thickness of the testis capsule. Spermatogonia cells slouching in the seminiferous tubule lumens, and destruction of sertoli cells, necrosis of spermatogonia, and macrophage infilteration in the seminiferous tubule lumen, degeneration and destruction of intermediate connective tissue cells, disorganization of spermatogonia and spermatocytes, Slouching of spermatocytes in the seminiferous tubule lumen, Congestion of blood vessels, Infilteration of inflammatory cells in the lumen of tubules. While, the E groups exhibit extremely typical seminiferous tubules, hyalinization of interstitial tissue with destruction of Leydig’s cells, normal capsule, some vacuoles or spaces between spermatocytes, virtually typical spermatogonia, few amount of sperms and relatively normal spermatocyte. In conclusion, Shilajit (Shi) acts synergistically in reducing MSG-induced testicular changes via anti-inflammatory and anti-degeneration effects.]]>
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