Akta Kimia Indonesia
Vol. 10 No. 2 (2025): Desember 2025

Skrining Fitokimia serta Uji Aktivitas Antioksidan Ekstrak Etanol 96% Daun Kelor (Moringa Oleifera, Lam) dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil)

Ni Luh Putu Junita Kusuma Dewi (Universitas Mahasaraswati Denpasar)
Ni Made sukma Sanjiwani (Unknown)
I Gusti Agung Ayu Kusuma Wardani (Universitas Mahasaraswati Denpasar)
I Wayan Surya Rahadi (Universitas Mahasaraswati Denpasar)
I Wayan Sudiarsa (Universitas PGRI Mahadewa Indonesia)
Ni Putu Ayu Mirah Mariati (Universitas Mahasaraswati Denpasar)



Article Info

Publish Date
15 Dec 2025

Abstract

All parts of the moringa plant, from the roots, seeds, stems to the leaves, are known to have medicinal properties. Its reported uses include anti-inflammatory, antioxidant, antibacterial, antifungal, and diuretic properties. This study focused on examining the presence of secondary metabolites and assessing the antioxidant potential of 96% ethanol extracts of moringa leaves (Moringa oleifera, Lam). The type of research conducted was a laboratory experiment. Moringa leaves were extracted using the Ultrasound-Assisted Extraction (UAE) method with 96% ethanol as the solvent. The resulting extract was then evaporated using a rotary evaporator to obtain a concentrated extract of 34.8 grams. The secondary metabolite content was evaluated through phytochemical screening, and the antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The antioxidant strength was determined by calculating the IC₅₀ value, which indicates the percentage of free radical scavenging by the test sample, and by the AAI value, which describes the antioxidant activity index of the extract. Based on the phytochemical screening results, the 96% ethanol concentrated extract of moringa leaves contained secondary metabolite compounds such as saponins, alkaloids, tannins, and steroids. The IC₅₀ value obtained from the extract was 75.428 ppm with an AAI of 0.530. As a comparison, ascorbic acid showed an IC₅₀ of 14.295 ppm and an AAI of 2.798.

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Journal Info

Abbrev

kimia

Publisher

Subject

Chemistry

Description

Akta Kimia Indoneisa publishes peer-reviewed open access articles in all areas of chemistry, including: Analytical Chemistry Physical Chemistry Organic Chemistry Inorganic Chemistry Biological Chemistry To submit an article, you need to register first. Please click register in the menus ...