<![CDATA[The use of non-degradable plastics as packaging has led to significant environmental issues. Waste generated from non-degradable plastics is resistant to degradation and contributes to environmental pollution. The application of biodegradable plastics offers a potential solution to this problem. Polyhydroxybutyrate (PHB) is an environmentally friendly bioplastic that exhibits advantageous properties compared to conventional plastics. PHB is a thermoplastic polyester polymer that can be degraded by microorganisms. The synthesis of PHB can be achieved through three main routes: synthetic polymerization, genetic engineering, and microbial fermentation. This study aimed to determine the yield of PHB synthesized via batch microbial fermentation. The microorganism employed in this study was Bacillus megaterium, which utilized substrates such as cheese whey, sugarcane molasses, glucose, and glycerol as nutrient sources for PHB biosynthesis. The highest PHB yield, 8.29 g/L, was obtained when cheese whey was used as the substrate. These results indicate that cheese whey provides an excellent nutrient source for PHB production.]]>
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