Indonesian Journal of Jamu
Vol. 11 No. 1 (2026): Jurnal Jamu Indonesia

Comparative Analysis of Total Flavonoid Content and Antioxidant Activity in Medicinal Plant Leaf Extracts Using Ethanol and Citric Acid

Aziz, Syaikhul (Unknown)
Mulyani, Isna (Unknown)
Nadiah, Syaadatun (Unknown)



Article Info

Publish Date
06 Feb 2026

Abstract

Flavonoids are bioactive compounds with antioxidant activity, and their extraction efficiency is strongly influenced by solvent selection. This study compared total flavonoid content and antioxidant activity of leaf extracts from Persea americana, Annona muricata, Piper betle, and Pandanus amaryllifolius obtained using ethanol and citric acid. Total flavonoid content was determined using the AlCl₃ spectrophotometric method with quercetin as the reference standard, while antioxidant activity was evaluated by the DPPH assay. Ethanol generally yielded higher flavonoid levels, with the highest content observed in P. americana leaves (3.87 mg QE/g), whereas citric acid extracts exhibited stronger antioxidant activity, reaching 94.21% DPPH inhibition in P. betle leaves. No significant correlation was observed between total flavonoid content and antioxidant activity. Overall, these results indicated that solvent selection critically determines extraction outcomes, and citric acid represents a viable green alternative for antioxidant-oriented applications.

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Journal Info

Abbrev

JJI

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

Description

Jurnal Jamu Indonesia (JJI) is dedicated to the exchange of information and widespread scientific understanding regarding the development of the world of herbal medicine through the publication of scientific papers. The themes of scientific papers within the scope of JJI include the theme of herbal ...