ILMU KELAUTAN: Indonesian Journal of Marine Sciences
Vol 31, No 1 (2026): Ilmu Kelautan

Utilization of COI Marker for Species Identification and Population Delineation of White Shrimp in the Demak Waters, Indonesia

Jasiel Junior Karosekali (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro)
Nenik Kholilah (Program Study of Marine Science, Faculty of Agriculture, University of Mataram)
Almay Atsiil Harits Syam (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro)
Subagiyo Subagiyo (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro)
Diah Permata Wijayanti (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro)
Muggi Bachtiar (Graduate School of Engineering and Science, University of The Ryukyus)



Article Info

Publish Date
01 Mar 2026

Abstract

White shrimp is one of the largest artisanal capture fisheries commodities in Demak. The utilization of these shrimps often overlooks their species category as they are mostly white in appearance. Growing trend of shrimp fishing may affect shrimp management due to population declination to the genetic level since the genetic data are insufficient. Hence, we aim to identify and study the genetic diversity of white shrimp caught in Demak Waters to provide an overview as a reference for fishery improvement project. A random purposive sampling method was used to collect a total of 90 white shrimp specimens from four fishing grounds (Babalan, Gojoyo, Menco, and Seklenting) in the Demak Waters. Specimens were extracted using Chelex 10%, amplified using PCR, and sequenced by the Sanger method based on the mtDNA COI gene. This research revealed the presence of five species of white shrimp: Penaeus merguiensis, Fenneropenaeus penicillatus, Penaeus vannamei, Metapenaeus brevicornis, and Metapenaeus ensis. The genetic diversity studies were continued using the Penaeus merguiensis species only. The results showed 17 haplotypes with a genetic diversity (Hd) of 0.6936 and a nucleotide diversity of 0.00108, which express moderate genetic diversity. Population analysis using Analysis of Molecular Variance (AMOVA) indicated a non-significant difference between the four study populations (panmictic population) with an FST value of 0.00756 (P-value < 0.05). Based on these results, an adequate management of stock is important. For instance, an open-closed season is needed in order to sustain and reduce capture pressure on the white shrimp population in Demak waters.

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Journal Info

Abbrev

ijms

Publisher

Subject

Earth & Planetary Sciences

Description

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and ...