Phytochemical Profile, Antioxidant Activity, and Hematological and Liver–Kidney Serum Markers of Lampung Green Robusta Coffee Extract in Lead-Induced Mice

Khairani, Iffa Afiqa
Ananta, Jeni Putri
Firmansyah, Kiki
Anisa, Hida Arliani Nur
Riana, Elisa Nurma
Mulyana, Jeane Siswitasari
Fatriani, Rizka

Indonesian Journal of Jamu
Vol. 11 No. 2 (2026): Jurnal Jamu Indonesia (In Progress)

Lampung Province is one of Indonesia’s major robusta coffee–producing regions, and green coffee beans contain bioactive compounds with potential biological benefits. This study analyzed the phytochemical composition and antioxidant activity of ethanolic extract of Lampung green robusta coffee beans and evaluated its effects on hematological parameters and liver–kidney serum markers in lead acetate–induced mice. Phytochemical screening revealed alkaloids, flavonoids, phenolics, saponins, tannins, and terpenoids. Quantitative analysis showed total phenolics of 122.76 mg GAE/g extract, flavonoids of 12.23 mg QE/g extract, tannins of 208.86 mg TAE/g extract, and saponins of 1.32%. The extract exhibited very strong antioxidant activity (IC₅₀ = 21.99 ppm). Lead acetate exposure (20 mg/kg BW for 14 days) decreased erythrocyte counts and increased leukocyte and urea levels. Administration of the extract (400 mg/kg BW) improved erythrocyte counts, modulated leukocyte levels, and reduced urea concentrations.