Asian Journal of Agriculture
Vol. 10 No. 1 (2026)

Anatomical compatibility of avocado grafting at different scion growth stages

RAMDAN HIDAYAT (Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur. Jl. Rungkut Madya No. 1, Gn. Anyar, Surabaya 60294, East Java, Indonesia)
ANTONIA JESSICA SHERLYN DA COSTA (Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur. Jl. Rungkut Madya No. 1, Gn. Anyar, Surabaya 60294, East Java, Indonesia)
JULI SANTOSO PIKIR (Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur. Jl. Rungkut Madya No. 1, Gn. Anyar, Surabaya 60294, East Java, Indonesia)
MUHAMMAD RIZKY ROMADHON (Department of Soil Science, Faculty of Agriculture, Universitas Lambung Mangkurat. Jl. Ahmad Yani Km. 36, Banjarbaru 70722, South Kalimantan, Indonesia)
OKKY TALITHA (Graduate School of Natural Science and Technology, Gifu University. Gifu 501-1193, Japan)
TILMIIDZAH SALMA FATHIN (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia)



Article Info

Publish Date
12 Apr 2026

Abstract

Abstract. Hidayat R, Da Costa AJS, Pikir JS, Romadhon MR, Talitha O, Fathin TS. 2026. Anatomical compatibility of avocado grafting at different scion growth stages. Asian J Agric 10 (1): g100131. https://doi.org/10.13057/asianjagric/g100131. This research aimed to evaluate the effectiveness of three grafting methods: cleft, wedge, and splice combined with three scion physiological stages: flushing (10 days after shoot emergence), endodormancy (30 days), and ecodormancy (50 days). The experiment assessed graft success in terms of survival rate, bud break duration, leaf production, flush interval, and vascular tissue compatibility. The splice grafting method using scions at the ecodormancy stage yielded the highest graft survival (72.50%), significantly outperforming other combinations. This treatment also achieved the earliest bud break (6.17 days), produced the most leaves (8.92 leaves per scion), and exhibited the shortest flush interval. Microscopic analysis at 45 and 60 days after grafting (DAG), using an Olympus CX21 binocular microscope equipped with an Optilab camera, revealed superior tissue compatibility in this treatment, characterized by minimal necrosis and successful union of xylem and phloem between the rootstock and scion. It is the first study to integrate physiological scion staging with histological validation of graft compatibility in avocado (Persea americana). These findings demonstrate that splice grafting with ecodormant scions significantly enhances morphological performance and vascular integration, providing a practical and scientifically supported protocol for improving avocado propagation in tropical highland conditions.

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Journal Info

Abbrev

aja

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Aims and Scope Asian Journal of Agriculture encourages submission of manuscripts dealing with all aspects to optimizing the quality and quantity of both plant and animal yield and final products, including agricultural economics and management, agricultural engineering and mechanization, agronomy ...