<![CDATA[Medical waste is hazardous and toxic waste (B3) produced by health care facilities and poses a risk of negative impacts on health and the environment if not managed according to procedures. This study aims to analyze the relationship between knowledge, length of service, and availability of facilities with the compliance of officers in medical waste management. This study used a cross-sectional design with a quantitative approach. The study population consisted of all 71 staff members at the South Thousand Islands Community Health Center, with a sample of 60 respondents meeting the inclusion criteria. Data were collected using a questionnaire, then tested for validity and reliability. Data analysis was performed using univariate and bivariate analysis with the Chi-Square test. The results of the study showed that knowledge was associated with staff compliance in medical waste management with a p-value of 0.000, length of service was associated with staff compliance in medical waste management with a p-value of 0.001, and availability of facilities was associated with staff compliance in medical waste management with a p-value of 0.000. Conclusion: There is a relationship between knowledge, length of service, and availability of facilities with staff compliance in medical waste management at the Kepulauan Seribu Selatan 2025 Community Health Center. Recommendation: The Kepulauan Seribu Selatan Community Health Center should conduct training or seminars for staff related to medical waste management]]>
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