Journal of Environmental Engineering and Sustainable Technology
Vol 4, No 1 (2017)

ISOLATION AND PROFILLING OVIDUCT SPECIFICT GLYCOPROTEIN IN OVIDUCTAL FLUID OF KACANG GOAT (Capra aegagrus hircus)

Herlina Pratiwi (FKH UB)



Article Info

Publish Date
19 May 2017

Abstract

Frozen semen supplemented by Oviduct Specific Glycoprotein (OVGP) is a new way to solve the decrease of in vitro fertility on goats caused by polyspermy and to increase the efficiency of artificial insemination on goats. Oviduct Specific Glycoprotein has associated with zona pellucida and localized in perivitelline space. The existence of Oviduct Specific Glycoprotein in perivitelline space may be linked to a mechanism that blocks polyspermic through vitelin block. Oviduct Specific Glycoprotein can be found in the fluid that was secreted by the oviduct (oviductal fluid). This study was aimed to determine Oviduct Specific Glycoprotein profile in the oviductal fluid of Kacang goats Malang district, West Java Province, Indonesia. Oviduct Specific Glycoproteins were collected from an oviductal liquid of kacang goats and samples were loaded to 12% SDS-PAGE to determine the weight of the molecule of Oviduct Specific Glycoprotein, further confirmed by western blot. The result of this study showed that molecule weight of Oviduct Specific Glycoprotein been collected from oviductal liquid was approximately 55-65 kDa. After being confirmed by western blotting the molecule weight of Oviduct Specific Glycoprotein was 65 kDa.

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Journal Info

Abbrev

jeest

Publisher

Subject

Control & Systems Engineering Decision Sciences, Operations Research & Management Energy Environmental Science Mechanical Engineering

Description

JEEST is an interdisciplinary and refereed journal, addresses matters related to environmental engineering and sustainable technology. Its range of themes encompasses ecological studies, field research, empirical work and descriptive analyses on topics such as environmental systems, environmental ...