<![CDATA[Background : Ultraviolet rays and pollution cause premature aging of the skin, darkening, dryness, and decreased elasticity. Parijoto fruit (Medinilla speciosa) offers potential for development as an anti-aging agent. This study aims to determine the inhibitory activity of elastase and tyrosinase enzymes from extracts and fractions of parijoto fruit in vitro, and to obtain the active fraction. Extraction was carried out by maceration using 96% ethanol solvent. Methods : Four fractions were obtained through liquid-liquid partition using n-hexane, ethyl acetate, butanol, and water solvents. Elastase and tyrosinase enzyme inhibition tests were carried out in vitro using an ELISA (multi-mode reader). The reference compound in the elastase enzyme inhibition test was gallic acid, and kojic acid was used to inhibit the tyrosinase enzyme. Results: The elastase enzyme inhibitory activity of the extract and four fractions and gallic acid with a concentration of 133.33 μg/mL were 13.28; 21.56; 14.88; 15.12; 11.24; and 21.83%. The tyrosinase inhibitory activity of parijoto fruit extract and four fractions and kojic acid with a concentration of 125 μg/mL were 17.01; 19.2; 23.96; 14.76; 18.20; 34.71%, respectively. Parijoto fruit extract and fractions had elastase and tyrosinase inhibitory activity. Conclusions: The n-hexane fraction was the active fraction in inhibiting the elastase enzyme by 21.56%, while the active fraction in inhibiting the tyrosinase enzyme was the ethyl acetate fraction which provided an activity of 23.96%.]]>
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