<![CDATA[Background : Ultraviolet rays and pollution cause premature aging of the skin, darkening, dryness, and decreased elasticity. Parijoto fruit (Medinilla speciosa) offers potential for development as an anti-aging agent. Methods : The current research was conducted to investigate the influence of Extended Pharmaceutical Marketing Mix (product, price, place, promotion, pharmacist, process and physical evidence) on customer satisfaction at pharmacies in Jember. . Four fractions were obtained through liquid-liquid partition using n-hexane, ethyl acetate, butanol, and water solvents. Elastase and tyrosinase enzyme inhibition tests were carried out in vitro using an ELISA (multi-mode reader). The reference compound in the elastase enzyme inhibition test was gallic acid, and kojic acid was used to inhibit the tyrosinase enzyme. Results: The elastase enzyme inhibitory activity of the extract and four fractions and gallic acid with a concentration of 133.33 μg/mL were 13.28; 21.56; 14.88; 15.12; 11.24; and 21.83%. The tyrosinase inhibitory activity of parijoto fruit extract and four fractions and kojic acid with a concentration of 125 μg/mL were 17.01; 19.2; 23.96; 14.76; 18.20; 34.71%, respectively. Parijoto fruit extract and fractions had elastase and tyrosinase inhibitory activity. Conclusions: The n-hexane fraction was the active fraction in inhibiting the elastase enzyme by 21.56%, while the active fraction in inhibiting the tyrosinase enzyme was the ethyl acetate fraction which provided an activity of 23.96%.]]>
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