Indigofera zollingeriana as an Alternative Feed and Immunostimulant For Snakehead Fish Infected with Aeromonas hydrophila

Siti Lestari (Freshwater Aquaculture Study Program, Rejang Lebong State Community Academy, Jl Basuki Rahmat No27 Dwi Tunggal, Curup, Kabupaten Rejang Lebong, Bengkulu, Indonesia)
Rizki Eka Puteri (Fisheries Science Program, Faculty Of Agriculture, South Sumatra University, Jalan Letnan Murod Nomor 55, Talang Ratu, Palembang, Indonesia)
Rani Ria Rizki (Fisheries Science Program, Faculty Of Agriculture, South Sumatra University, Jalan Letnan Murod Nomor 55, Talang Ratu, Palembang, Indonesia)
Guttifera (Fisheries Science Program, Faculty Of Agriculture, South Sumatra University, Jalan Letnan Murod Nomor 55, Talang Ratu, Palembang, Indonesia)
Donny Prariska (Fisheries Science Program, Faculty Of Agriculture, South Sumatra University, Jalan Letnan Murod Nomor 55, Talang Ratu, Palembang, Indonesia)
Raudhatus Sa'adah (Food Agribusiness Study Program, Department of Agricultural Technology and Business Engineering, Sriwijaya State Polytechnic, Jl. Srijaya Negara Bukit Lama, Palembang City)
Febby Hadi Setyawan (Aquaculture Study Program, Faculty of Agriculture, Samudra University, Jl. Prof.Dr.Syarief Thayeb, Meurandeh, Kec. langsa, Langsa City, Aceh, Indonesia)
Ramanda Ahmad Rizal Rifa'i (Aquaculture Study Program, Faculty of Fisheries and Marine Science, Mulawarman University, Jl. Gn. Tabur, Gn. Kelua, Kec. Samarinda Ulu, Samarinda City, East Kalimantan)

Grouper: Jurnal Ilmiah Perikanan
Vol. 17 No. 1 (2026): Grouper: Jurnal Ilmiah Perikanan

The demand for snakehead fish in South Sumatra continues to increase, encouraging farmers to develop intensive cultivation. However, the main challenges of high feed costs and disease attacks need to be addressed to maintain optimal productivity. One alternative being studied is the use of Indigofera zollingeriana as a source of protein and immunostimulant in feed. This study aims to evaluate the effect of substituting fish meal with Indigofera flour on the immune response of snakehead fish (Channa striata) infected with Aeromonas hydrophila bacteria. The study used a Completely Randomized Design with four treatments of fish meal substitution with Indigofera zollingeriana flour, namely 0%, 15%, 30%, and 45%. The parameters observed included phytochemical tests, growth, blood profile, and water quality. The results of the phytochemical test showed the presence of flavonoids, alkaloids, tannins, and saponins. In the blood profile, the 45% treatment produced the highest erythrocyte value (4.54 × 10⁴ cells) and hematocrit of 38%. The best growth was shown in the 30% treatment with an SGR value of 2.56±0.23d and an FCR of 1.61±0.11a, while the highest survival rate (84.44%) was obtained in the 45% treatment. These findings indicate that Indigofera has the potential as a functional feed to improve the immunity and productivity of snakehead fish.