Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)
Vol. 15 No. 1 (2026): February 2026

Effect of Solvent Type on the Antibacterial Activity of Trigona laeviceps Propolis Extract

Intan Nurul Azni (IPB University)
Dede Robiatul Adawiyah (IPB University)
Uswatun Hasanah (IPB University)
Nancy Dewi Yuliana (IPB University)



Article Info

Publish Date
20 Feb 2026

Abstract

Propolis is a resinous compound which is mixed with saliva, wax, and other metabolic products of bees. One of the benefits of propolis is as an antimicrobial agent. The objective of this research was to determine antibacterial activity Trigona laeviceps bees propolis originating from Bogor West Java extracted with various solvents (water, ethanol, hexane). The tested bacteria consist of 2 Gram negative and 5 Gram positive bacteria. The raw propolis were characterized for gum, tannin, flavonoid, alkaloid, saponin, total phenol content, and proximate composition. Antibacterial activity was tested using the disc diffusion method. The data were analyzed using the ANOVA test and further tested with the Duncan test at a significance level of 5%. The results showed that the largest component of the sampel was lipid (65.47%). Raw propolis contains phenol (3.08 mg/g), tannin (34.45 mg/g), and flavonoid (21.88 mgQCE/g). The highest yield of propolis extract was obtained with hexane solvent (51.03%), followed by ethanol (18.17%), and water (15.58%). All propolis extracts did not have an inhibition zone against Gram-negative bacteria, but did for Gram-positive bacteria. The propolis extract using ethanol showed the highest diameter inhibition zone (16.92 mm). The finding of this study may be utilized to improve Trigona laeviceps propolis quality in Bogor.

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Journal Info

Abbrev

JTP

Publisher

Subject

Agriculture, Biological Sciences & Forestry Engineering

Description

Jurnal Teknik Pertanian Lampung or Journal of Agricultural Engineering (JTEP-L) is a peer-reviewed open-access journal. The journal invites scientists and engineers throughout the world to exchange and disseminate theoretical and practice-oriented researches in the whole aspect of Agricultural ...