ISOLATION AND STRUCTURAL ELUCIDATION OF FLAVONOID FROM BOESENBERGIA PANDURATA RHIZOME

Moch Abdussalam (Universitas YARSI)
Juniarti (Biochemistry Departement Faculty of Medicine, Yarsi University, Central Jakarta, 10520)
Indah Permata Yuda (Research Centre,Yarsi University, Central Jakarta, 10520)

Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya
Vol. 8 No. 1 (2026): Literasi Artikel Penelitian Kimia

Temu Kunci (Boesenbergia pandurata) rhizome constitutes a botanical resource long harnessed for ethnomedicinal applications among localized populations. To validate its long-standing traditional application, this investigation aimed to characterize the bioactive chemical profile of the Boesenbergia pandurata rhizome. The dried, pulverized rhizome was initially subjected to maceration for crude extraction of secondary metabolites, followed by a meticulous purification sequence. Vacuum Liquid Chromatography (VLC) was first employed for bulk separation, and subsequent Radial Chromatography refined the flavonoid-rich fractions to yield a pure isolate. The structure of this isolate was then conclusively resolved using advanced 1H-NMR and 13C-NMR spectroscopy, which unambiguously identified the primary compound as pinostrobin.