The increasing of the callus formation of cashew (Anacardium occidentale L.) from leaf explants and the petals have successfully done through the use of a combination of plant growth regulators 2,4-D, pocloram and BA. On leaf explants the highest percentage of callus formation was obtained in combination treatment of plant growth regulators 2,4-D 5 mg/l + BA 1 mg/l and 2,4-D 5 mg/l + picloram 0.5 mg/l with callus formation percentage of 33.3%. On the use of petal explants the highest percentage of callus formation was obtained on 2,4-D treatment of 10 mg/l with callus formation percentage of 40%. Formed callus were blackish brown demonstrate the use of PVP 200 mg/l still can not overcome the problem of phenol oxidation.
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