Jurnal Insan Farmasi Indonesia
Vol 9 No 1 (2026): Jurnal Insan Farmasi Indonesia

Analisis Kualitatif dan Kuantitatif Senyawa Polifenol dan Glikosida pada Fraksi Ekstrak Etanol Daun Jambu Mete (Anacardium occidentale L.) Menggunakan Spektrofotometri UV-Vis

Rabiatul Adawiyah (Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin)
Evita Wulandari (Akademi Farmasi Tadulako Farma)
Nur Ani (Akademi Farmasi Tadulako Farma)



Article Info

Publish Date
30 May 2026

Abstract

Cashew leaves (Anacardium occidentale L.) are known to contain secondary metabolites with potential medicinal properties, including polyphenols and glycosides. This study aimed to determine the qualitative and quantitative contents of polyphenol and glycoside compounds in the ethanol extract fractions of cashew leaves using the UV-Vis spectrophotometric method. Cashew leaves were extracted using 96% ethanol. Fractionation was carried out using n-hexane and ethyl acetate solvents. Glycoside content was determined using quercetin as the standard at a wavelength of 430 nm, while polyphenol content was determined using gallic acid as the standard at a wavelength of 700 nm. Qualitative test results showed that both n-hexane and ethyl acetate fractions were positive for glycosides, indicated by a green color, and positive for polyphenols, indicated by a greenish-purple color. Quantitative results showed that the glycoside content in the n-hexane fraction was 3.999 mgQE/g and in the ethyl acetate fraction was 8.043 mgQE/g. The polyphenol content in the n-hexane fraction was 2.138 mgGAE/g and in the ethyl acetate fraction was 3.198 mgGAE/g. The ethyl acetate fraction exhibited higher glycoside and polyphenol contents compared to the n-hexane fraction.

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Journal Info

Abbrev

JIFI

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Physics

Description

Focus and Scope Jurnal Insan Farmasi Indonesia (JIFI) is a broad-based primary journal covering all branches of pharmacy and its sub-disciplines that contains complete research articles, short communication and review articles. JIFI is a forum for the publication of quality and original works that ...