Background: Pregnant women in the second trimester experience physical and hormonal changes, as well as increased insulin resistance, which may lead to elevated blood glucose levels. If not detected early, these conditions may increase the risk of developing gestational diabetes mellitus. Objective: To determine the blood glucose levels of pregnant women in the second trimester at 24–28 weeks of gestation in order to detect the risk of gestational diabetes mellitus. Methods: This study employed a quantitative descriptive design. The population consisted of 37 pregnant women, with a total of 30 respondents selected through purposive sampling. The inclusion criteria were pregnant women in the second trimester with a gestational age of 24–28 weeks. Data collection was conducted from March 6 to March 31, 2024. The research instruments included a structured interview questionnaire consisting of factors influencing gestational diabetes mellitus and an observation sheet. Results: The results showed that 10% of pregnant women were classified as having prediabetes, while 90% had random blood glucose levels within the normal range. Blood glucose levels were influenced by factors such as age, history of diabetes mellitus, overweight status, history of polycystic ovary syndrome (PCOS), and consumption of sweet foods. Implications: Early detection is necessary to identify gestational diabetes mellitus beginning in the early trimester of pregnancy, along with regular antenatal care (ANC) examinations Keywords: Blood Glucose; Pregnant Women; Gestation Age 24-28 Weeks
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