Background: Vaginal discharge is a reproductive health disorder, one of the causes of which is Candida sp. fungal infection. The growth of this fungus can be influenced by genital area hygiene and supporting environmental factors. Objective: determine the frequency distribution of Candida sp. in the urine of female students at University X in 2026. Research Design: using descriptive, the research sample was 36 respondents using the Sampling Technique, namely Simple Random Sampling. Method: Microscopic KOH 20%. Results: shows that of the 36 respondents studied, it is known that the results of the examination for the presence of Candida sp. in the urine of female students at University X were all negative, namely 36 respondents (100%). Based on personal hygiene, of the 36 respondents, it shows that all respondents (100%) are included in the good category with negative Candida sp. examination results. Based on the use of pantyliners, 9 respondents (100%) used pantyliners and 27 respondents (100%) did not use pantyliners, with all examination results showing negative. Based on antiseptic use, 13 respondents (100%) used antiseptic and 23 respondents (100%) did not use antiseptic, with all test results also negative. Based on water use, all respondents (100%) used running water and all showed negative results for Candida sp. Suggestion: For further researchers, it is recommended to use more specific examination methods such as culture with Sabouraud Dextrose Agar media. Keywords: Candida sp, Urin, Microscopic KOH 20%.
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