Background: Respiratory infections, including pneumonia, are a leading cause of global mortality. These infections can be caused by various pathogens, including typical and atypical bacteria, as well as viruses, fungi, and parasites. Rapid and accurate diagnosis is crucial, especially in cases requiring intensive care, to ensure timely and appropriate treatment. Objective: This research aimed to obtain an overview of the application of the FilmArray method, a nested multiplex pcr base method, in determining the diagnosis and management of respiratory tract infections. Methods: This study was a cross-sectional study with a descriptive analytic design on patients with respiratory tract infections. The study was conducted from April to June 2022 at the Biomolecular Laboratory, Laboratory Installation of RSPI Prof. Dr. Sulianti Saroso. Results: Of the 72 patients examined, 27 (37.5%) tested positive for one of the viruses, while 45 (62.5%) tested positive for neither viruses nor bacteria. Among the positive patients, the most commonly detected virus was human rhinovirus/enterovirus (48%), followed by SARS-CoV-2 (48%), and human metapneumovirus (4%). No atypical bacteria or a double infection of virus and bacteria were detected. Conclusion: This research indicates that molecular-based methods using the FilmArray respiratory panel can be used for the rapid and accurate detection of respiratory tract infection cases, especially those caused by respiratory viruses and atypical bacteria.
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