Journal of Healthcare and Biomedical Science (JHBS)
Vol. 4 No. 2 (2026): Journal of Healthcare and Biomedical Science (JHBS)

Development, Characterization, and Comparative Analysis of Halal-Compliant Fortified Caulerpa lentillifera Jelly with Enhanced Nutritional and Antioxidant Properties

Nor Atikah Husna Ahmad Nasir (Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia
2Faculty of Applied Sciences, Universiti Teknologi MARA)

Mohd Hafis Yuswan (1Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia)
Nor Nadiah Abd Karim Shah (Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia
3Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)

Kaiser Mahmood (1Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia)
Aswir Abd Rashed (Nutrition Unit, Institute for Medical Research, National Institutes of Health)
Kazunori Kadota (School of Pharmaceutical Sciences, Wakayama Medical University)
Yus Aniza Yusof (Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia
3Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)



Article Info

Publish Date
30 Jun 2026

Abstract

Oxidative stress is linked to many chronic diseases, making antioxidant-rich functional foods important for health. This study aims to evaluate the nutritional composition and antioxidant capacity of fortified C. lentillifera jelly (FJ), control jelly (CJ), and commercial jelly (MJ) using mineral analysis, proximate analysis, and antioxidant assays, followed by Principal Component Analysis (PCA). The fortified jelly, formulated without gelatin or other non-Halal ingredients, ensures compliance with Halal dietary requirements while maintaining desirable product characteristics. The results showed that FJ exhibited enhanced antioxidant activity, with DPPH and ABTS scavenging capacities of 68% and 45%, respectively. These antioxidant activities indicate the ability of the fortified jelly to scavenge free radicals, which are implicated in cellular damage and oxidative stress-related diseases. In addition, FJ had lower sodium content than MJ, suggesting its potential as a healthier alternative. The relatively lower moisture content in FJ (50.34%) compared to MJ (88.28%) contributed to a higher concentration of nutrients and carbohydrates within the product matrix. PCA explained 97.4% of the total variance, clearly differentiating the jelly samples based on their compositional attributes. PC1 was primarily associated with potassium, fat, and ash, while PC2 was influenced by antioxidant activity and selected minerals, including magnesium and zinc, which were more prominent in FJ. Overall, these findings suggest that C. lentillifera-fortified jelly may help reduce oxidative stress and support better health as a Halal functional food.

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Journal Info

Abbrev

jhbs

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Description

Journal of Healthcare and Biomedical Science (JHBS) is an open access, peer-reviewed journal that encompasses all publication of novel research in healthcare and biomedical sciences, with an emphasis on the molecular studies of biomedical problems and molecular mechanisms. The scope of this journal ...