Thalassiosira sp. is a significant marine diatom utilized as live feed in aquaculture; however, its culture productivity frequently remains below optimal levels. The present study evaluated the growth performance and culture quality of Thalassiosira sp. under two parallel cultivation strategies: a mixotrophic system supplemented with sucrose and a system supplemented with Indole-3-Acetic Acid (IAA). Both cultivation schemes were maintained for seven days under identical environmental conditions. Sucrose was administered at concentrations ranging from 0.25 to 1 g/L, while IAA was applied at 2.5 to 10 mg/L. Cell density, specific growth rate, dry biomass, and total Vibrio count were measured. Sucrose supplementation, particularly at 1 g/L, resulted in increased growth and biomass accumulation but was accompanied by elevated Vibrio abundance. IAA supplementation also promoted growth and biomass production, with the most pronounced effect observed at 10 mg/L, although it did not reduce bacterial proliferation. These findings indicate that both sucrose and IAA can enhance Thalassiosira sp. culture productivity under controlled conditions, but effective microbial management remains essential for practical implementation.
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