<![CDATA[Acclimatization is a critical stage in the propagation of orchids produced through tissue culture, as plantlets must adapt from in vitro conditions to the ex vitro environment. The use of symbiotic microorganisms has the potential to enhance acclimatization success by improving nutrient uptake and tolerance to environmental stress. This study aimed to analyze the effect of symbiotic bacterial application on the success of orchid plantlet acclimatization. The experiment employed a Completely Randomized Design (CRD) with five concentrations of symbiotic bacteria: no bacteria (A0), 2% (A1), 4% (A2), 6% (A3), and 8% (A4), each with ten replications. Observed parameters included survival rate, plantlet height, root length, and total chlorophyll content. The results showed that the application of symbiotic bacteria significantly affected all observed parameters. Treatment A3 produced the highest growth and survival rate, reaching 90%.]]>
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