Canrea Journal: Food Technology, Nutritions, and Culinary Journal
VOLUME 9 ISSUE 1, JUNE 2026

Fortification of cow and soymilk kefir with pomegranate peel extracts: Impact on physicochemical, microbial viability, and sensory properties

Imam Adriansyah (Study Program of Food Technology, Faculty of Engineering, Universitas Bumigora, Mataram, Indonesia)
Mohamed Said Boulkrane (Study Program of Food Technology, Faculty of Biotechnology, ITMO University, Saint Petersburg, Russia)
Sri Handayani Nofiyanti (Study Program of Agricultural Engineering and Biosystem, Faculty of Agricultural Technology, Universitas Udayana, Bali, Indonesia)
Darus Dina Imama (Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Laila Fathiyaturrahmi (Study Program of Statistics, Faculty of mathematics and natural science, Universitas Mataram, Mataram, Indonesia)



Article Info

Publish Date
28 Jun 2026

Abstract

Kefir is a fermented beverage valued for its probiotic-associated properties, although its antioxidant capacity remains relatively moderate. Pomegranate peel, a phenolic-rich agro-industrial by-product, offers potential as a sustainable fortifying ingredient for functional beverage development. This study investigated the effects of pomegranate peel extract (PPE) addition (0–20%, w/v) on the physicochemical, microbiological, antioxidant, and sensory characteristics of cow milk and soymilk kefir. Kefir samples were prepared using a commercial starter culture and evaluated for total phenolic content (TPC), antioxidant activity, pH, viscosity, viable lactic acid bacteria (LAB) counts, and sensory acceptance. PPE fortification significantly increased (p < 0.05) TPC and antioxidant activity in both kefir matrices. TPC increased from 55.31 to 182.87 mg GAE/g in cow milk kefir and from 60.91 to 199.29 mg GAE/g in soymilk kefir. LAB counts also increased substantially, with more than a two-fold increase in cow milk kefir and an approximately five-fold increase in soymilk kefir. PPE significantly reduced viscosity in cow milk kefir, while soymilk kefir remained rheologically stable, indicating a matrix-dependent response. Sensory evaluation revealed no statistically significant differences (p > 0.05) in taste, aroma, or color, although higher PPE levels tended to show lower numerical sensory scores, particularly in soymilk kefir. These findings indicate that PPE can enhance the phenolic content, antioxidant potential, and LAB viability of both dairy- and plant-based kefir. Moderate inclusion levels (5–15%) appeared to provide the most balanced functional and sensory profile, supporting the potential use of PPE as a natural fortifying ingredient in fermented beverage formulations.

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Journal Info

Abbrev

canrea

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Canrea Journal: Food Technology, Nutritions, and Culinary Journal (E-ISSN 2621-9468) merupakan publikasi ilmiah bidang pangan sebagai media komunikasi dan informasi yang mencakup aspek hulu dan hilir pangan yang diharapkan dapat berkontribusi terhadap perkembangan pangan dan intelektual. Artikel ...