<![CDATA[Anemia is a hematological condition characterized by decreased hemoglobin (Hb) levels, leading to impaired oxygen transport and reduced physiological function. Natural products such as kelakai leaves (Stenochlaena palustris) extract and stingless bee honey have potential as complementary therapies for anemia due to their iron content and bioactive compounds, including flavonoids and antioxidants that may support erythropoiesis. This study aimed to evaluate the effect of a combination of kelakai leaves extract and stingless bee honey on hemoglobin levels in mice (Mus musculus). A true experimental study with a pretest–posttest control group design was conducted using 30 male mice divided into six groups: normal control, untreated anemic control, anemic group treated with iron supplementation, and three treatment groups receiving combinations of kelakai leaves extract and stingless bee honey at ratios of 30:70, 50:50, and 70:30. Anemia was induced using sodium nitrite, and hemoglobin levels were measured before and after treatment. Data were analyzed using one-way analysis of variance (ANOVA) followed by post hoc tests. The results demonstrated that sodium nitrite induction successfully reduced hemoglobin levels below normal, while all treatment groups showed a significant increase in hemoglobin levels compared to the untreated anemic group (p < 0.05). The highest mean hemoglobin level was observed in the 70:30 combination group (16.36 g/dL), whereas the lowest was found in the untreated anemic group (10.22 g/dL). In conclusion, the combination of kelakai leaves extract and stingless bee honey effectively increased hemoglobin levels in mice, with the 70:30 ratio identified as the most effective formulation.]]>
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