Walisongo Journal of Chemistry
Vol. 9 No. 1 (2026): Walisongo Journal of Chemistry

EXTRACTION AND IDENTIFICATION OF COMPOUNDS FROM BIOETHANOL PRODUCTION WASTE WITH POTENTIAL AS PEST CONTROL AGENTS BY LC-MS/MS

Etyn Yunita (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jakarta, Indonesia)
Sumarlin La Ode (Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jakarta, Indonesia)
Dhea Aprilliyani (Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jakarta, Indonesia)
Yudi Harsono (CV. Resia Niaga, Cherry II St., Cijujung, Bogor, West Java, Indonesia)
Pamungkas Riski Ferdian (Research Center for Applied Zoology, Research Organization for Life Sciences and Environment, BRIN)
Anang Setiawan Achmadi (Museum Zoologicum Bogoriense, Research Center for Biosystematics and Evolution, Research Organization for Life Sciences and Environment, BRIN)
Nurhasni Nurhasni (Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jakarta, Indonesia)
Mia Azizah (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jakarta, Indonesia)



Article Info

Publish Date
20 Jul 2026

Abstract

Bioethanol production generates waste by-products that may contain various valuable chemical compounds, many of which have not yet been fully explored or utilized. Among these by-products, liquid waste, commonly known as vinasse, is one of the most frequently processed and reused forms. This study aimed to extract and identify compounds from bioethanol production waste, particularly those with potential as pest control agents. Chemical profiling was conducted using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Analysis of samples extracted with n-hexane, ethyl acetate, ethanol, and water revealed several putative bioactive compounds with pesticidal activity. Notable identified compounds included DEET (N,N-diethyl-m-toluamide) (m/z = 192.1388), loliolide (m/z = 197.1178), (S)-reticuline (m/z = 330.1705), 3,5,6-trihydroxy-5-(hydroxymethyl)-2-methoxy-2-cyclohexen-1-one (m/z = 206.0712), and cuscohygrine (m/z = 225.1983). These compounds are known to exert various biological activities, including antibacterial, antifungal, and insect-repellent effects, each operating through distinct mechanisms. However, further functional validation through field implementation studies is necessary to evaluate the effectiveness and long-term sustainability of their application, particularly regarding the presence of DEET in the samples.

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Journal Info

Abbrev

wjc

Publisher

Subject

Chemistry

Description

Walisongo journal of chemistry is a peer reviewed and open access journal published by Chemistry Department, faculty of Science and Technology, UIN Walisongo Semarang. This journal covering all areas of chemistry including inorganic, organic, physic, analytic, biochemistry, and environmental ...