The success of grapevine (Vitis vinifera L.) propagation through stem cuttings is strongly influenced by planting media quality during the nursery stage. Under tropical lowland conditions, unstable root-zone environments often reduce seedling vigor and biomass accumulation. Therefore, suitable manure-based media are needed to improve vegetative establishment and nursery efficiency. This study aimed to evaluate the effects of different manure-based planting media on vegetative growth and biomass accumulation of grapevine stem cuttings under tropical screen-house conditions in Bandar Lampung, Indonesia. The experiment was arranged in a randomized complete block design consisting of four treatments: M1 = sand + soil (1:1), M2 = sand + soil + cattle manure (1:1:1), M3 = sand + soil + goat manure (1:1:1), and M4 = sand + soil + chicken manure (1:1:1), with six replications. The results showed that the cattle manure-based medium consistently produced the best vegetative performance, increasing shoot length, leaf number, leaf area, fresh weight, and dry weight by 67.6%, 83.3%, 83.1%, 38.5%, and 40.4%, respectively, compared with the control treatment. Overall, cattle manure-based media provided the most favorable conditions for vegetative establishment and biomass accumulation of grapevine stem cuttings under tropical nursery conditions.
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