Jurnal Perikanan dan Kelautan
Vol. 31 No. 1 (2026): February

The Effect of Protease Enzyme and Citric Acid Addition on the Organoleptic Properties of Catfish Head Milk

Welly Deglas (Politeknik Tonggak Equator Pontianak)
Fransiska Fransiska (Politeknik Tonggak Equator Pontianak)
Maria Krisna Evania (Politeknik Tonggak Equator Pontianak)



Article Info

Publish Date
28 Feb 2026

Abstract

This study evaluated the effects of combining protease enzyme dosages (0.5%, 1%, and 1.5%) with citric acid levels (0.1%, 0.3%, and 0.5%) on the organoleptic quality of catfish head milk, including color, aroma, taste, homogenization/clarity, and panelist acceptance. The product was formulated through enzymatic hydrolysis under controlled pH conditions and subsequently assessed using a sensory scoring scale. The results showed that the treatments significantly affected color (F = 4.13; p < 0.05), homogenization (F = 2.39; p < 0.05), and overall hedonic preference (F = 3.03; p < 0.05), but had no significant effects on aroma (F = 1.62; p > 0.05) or taste (F = 1.39; p > 0.05). Specifically, the best color was obtained at 0.5% protease and 0.5% citric acid (A1B3), while the highest homogenization occurred at 0.5% protease and 0.1% citric acid (A1B1). In terms of overall liking, the highest score was recorded at 1.5% protease and 0.5% citric acid (A3B3), followed by 0.5% protease and 0.3% citric acid (A1B2). However, when considering the balance among all attributes (color, aroma, taste, and homogenization), the combination of low protease with low to moderate citric acid levels (0.5% protease; 0.1–0.3% citric acid) provided the most stable performance, producing a relatively bright color, more acceptable flavor and aroma, and better physical stability. Overall, the enzymatic hydrolysis approach with moderate pH adjustment proved effective at enhancing sensory acceptance and offers a promising method for transforming low-value fish by-products into consumer-preferred functional food products

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Journal Info

Abbrev

JPK

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

Description

Journal of Fisheries and Marine was published by the Faculty of Fisheries and Marine of the Universitas Riau in 2007. The scope of the research included: Aquaculture, fisheries socioeconomic, fisheries technology, management of aquatic resources, utilization of fisheries and marine ...