PharmaCine: Journal of Pharmacy, Medical and Health Science
Vol. 7 No. 1 (2026): PharmaCine: Journal of Pharmacy, Medical and Health Science

In Vitro Antioxidant Activity of Extracts and Fractions from Common Bean (Phaseolus vulgaris L.) Pods and Leaves Using the DPPH Method: In Vitro Antioxidant Activity of Extracts and Fractions from Common Bean (Phaseolus vulgaris L.) Pods and Leaves Using the DPPH Method

Dita Meidinata (Department of Pharmacy, Faculty of Science and Technology, Halim Sanusi University, Bandung 40271, Indonesia.)
Novian Liu Wion (Department of Pharmacy, Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi 40531, Indonesia)



Article Info

Publish Date
31 Mar 2026

Abstract

Phaseolus vulgaris L. is a legume rich in nutrients and containing a variety of chemical constituents. Although its seeds have been extensively studied, comparative information regarding the antioxidant potential of the pod and leaf particularly in various solvent fractions has not yet been established. The objective of this study was to determine the in vitro antioxidant activity of ethanol extracts, n-hexane fractions, ethyl acetate fractions, and aqueous fractions of the pods and leaves of common bean (Phaseolus vulgaris L.) using UV-Visible spectrophotometry with the 1,1-dipheny l-2-picrylhydrazyl (DPPH) method. Phytochemical screening results indicated that the pods and leaves simplicia of the common bean contain flavonoids, polyphenols, saponins, monoterpenoids, sesquiterpenoids, and quinones. The n-hexane fraction of common bean leaves had an EC50 value of 7.16 µg/mL, and the aqueous fraction of common bean pod had an EC50 value of 10.24 µg/mL, each exhibited the strongest free radical scavenging activity compared to the other extracts and fractions. These values were determined using quercetin as a reference substance, which had a concentration of 0.6321 µg/mL. This indicates that common bean pods and leaves have potential as natural sources of antioxidant compounds. Therefore, further research is needed to isolate the compounds that act as antioxidants in common bean pod and leaf extracts, as well as to study the in vivo antioxidant activity of common beans.

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Journal Info

Abbrev

pharmac

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Description

PharmaCine: Journal of Pharmacy, Medical, and Health Sciences is a peer-reviewed journal dedicated to scientific publications in the fields of pharmacy, medicine, and health sciences. PharmaCine is published biannually, in March and September, by the Undergraduate Pharmacy Program, Faculty of Health ...