Cow urine has the potential to become biourin. This study aims to determine the effect of the type of diffuser on the quality of biourin, as well as to determine the best type of diffuser during the fermentation process. This study used a completely randomized design consisting of 5 treatments and 3 replications. Each treatment contained 10 liters of urine, 2% nitrifying bacteria, and 2% molasses. The treatment was carried out without aeration, treatment using aeration without a diffuser, treatment using a fine bubble diffuser, treatment using a coarse bubble diffuser, and treatment using an aerated stone diffuser. This fermentation was carried out for 20 days with measurements every 5 days. Parameters observed were temperature, pH, TDS, EC, C-Organic, N-Total, and C/N Ratio during the fermentation process. The results of the study showed that the use of several types of diffusers had a significant effect on the process of fermenting cow urine. The fermentation process without using a diffuser showed that as the fermentation time increased, the pH value decreased from 7.72 to 7.54. On the 20th day of bio-urine fermentation, the highest Biochemical Oxygen Demand value was shown by the treatment using a fine bubble diffuser with a value of 9.2 mg/l. Of the five urine fermentation treatments, only fermentation with a diffuser met standard No.70/Permentan/SR.140/10/2011. Treatment with a type of fine bubble diffuser is the best treatment.
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