Indonesian Journal of Chemical Science and Technology
Vol. 9 No. 2 (2026): JULY 2026

Phytochemical Screening and Antioxidant Activity of Ethanolic Extracts of Young and Mature Butterfly Pea (Clitoria ternatea) Leaves Using the DPPH Method

Amelia Dwi Putri (State University of Medan)



Article Info

Publish Date
31 Jul 2026

Abstract

Butterfly pea leaves (Clitoria ternatea) are one part of the plant that contains various secondary metabolites and have the potential to serve as a natural antioxidant source. This study aimed to determine the phytochemical content and compare the antioxidant activity of ethanolic extracts of young and mature butterfly pea leaves using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The samples were extracted by maceration using 96% ethanol, followed by qualitative phytochemical screening and antioxidant activity testing at concentrations of 20, 40, 60, and 80 μg/mL. The results showed that both extracts were positive for phenolics, flavonoids, tannins, alkaloids, saponins, and steroids, but negative for triterpenoids. The IC₅₀ values of young leaf extract, mature leaf extract, and vitamin C were 80.408, 58.023, and 5.655 μg/mL, respectively. Based on the IC₅₀ values, both extracts were classified as strong antioxidants, while vitamin C was classified as a very strong antioxidant. The mature butterfly pea leaf extract showed higher antioxidant activity than the young leaf extract, indicating that leaf maturity affects the antioxidant potential of butterfly pea leaves.

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Journal Info

Abbrev

aromatika

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

Description

Jurnal ini mempublikasikan artikel di bidang Kimia yang meliputi penelitian dasar dan terapan. Bentuk Publikasi di Indonesian Journal of Chemical Science and Technology : * Artikel * Review (berdasarkan permintaan Dewan Redaksi) * Communication (berdasarkan permintaan Dewan ...