<![CDATA[Optimizing environmental factors in cultivating microalgae is essential to obtain a high biomass yield for biodiesel and other biomass related products production, which is a green renewable energy source to overcome the scarcity of fossil fuel energy in the future. Biodiesel can be produced using microalgal lipids in the form of Fatty Acid Methyl Esters (FAMEs). One of the promising strains is Euglena sp., an acidophilic microalga that produces various valuable bioproducts, including lipids, as biodiesel feedstocks. Here, we studied light intensity combined with 15% CO2 to enhance the production of FAMEs and other metabolites in a local strain of Euglena sp. under 500 lux, 2,100 lux, 4,500 lux, 6,000 lux, and 8,500 lux. We also evaluated its effect on the growth, biomass, and accumulation of primary and secondary metabolites, such as lipids, carbohydrates, proteins, FAME, and pigment contents. Based on this study, the maximum saturated, monounsaturated, and polyunsaturated acids were found in the 8,500 lux (42.345%), 500 lux (59.01%), and 4,500 lux (23.705%), respectively. The highest percentage of FAMEs was C16:1 or methyl palmitoleate (32.46%) found at 500 lux. However, the total FAMEs in 500 lux (13 FAMEs) were lower than those in the other treatments (24 FAMEs). Meanwhile, the highest biomass accumulation, specific growth rate, lipids, carbohydrates, and pigment contents such chlorophyll a, b, carotenoid were found in 6,000 lux. The results indicated that variations in light intensity with 15% CO2 injection resulted in specific differences in growth rate, productivity of primary and secondary metabolites, and fatty acid production in Euglena sp.]]>
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